PDXK Mouse Monoclonal Antibody [Clone ID: OTI5H5]

CAT#: CF502998

Carrier-free (BSA/glycerol-free) PDXK mouse monoclonal antibody, clone OTI5H5 (formerly 5H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDXK (RC200975, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDXK. Positive lysates LY418499 (100ug) and LC418499 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-PDXK monoclonal antibody (1:200).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT, Cat# LC810293T) and PDXK-Knockout HEK293T cells (KO, Cat# LC841087) were separated by SDS-PAGE and immunoblotted with anti-PDXK monoclonal antibody TA502998 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502998)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502998)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502998)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502998)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502998)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502998)

• Anti-PDXK mouse monoclonal antibody (TA502998) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDXK (RC200975).

• HEK293T cells transfected with either RC200975 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDXK antibody (TA502998), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5H5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDXK(NP_03672) produced in HEK293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.9 kDa
• Gene Name: Homo sapiens pyridoxal kinase (PDXK), transcript variant 1, mRNA.
• Database Link:
  NP_003672
  Entrez Gene 83578 RatEntrez Gene 216134 MouseEntrez Gene 8566 Human
  UniProt ID O00764
• Background: The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: C21orf97; C21orf124; HEL-S-1a; PKH; PNK; PRED79

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Vitamin B6 metabolism