Apg3 (ATG3) Mouse Monoclonal Antibody [Clone ID: OTI4F6]

CAT#: CF503370

Carrier-free (BSA/glycerol-free) ATG3 mouse monoclonal antibody, clone OTI4F6 (formerly 4F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG3 (RC203453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG3. Positive lysates LY411559 (100ug) and LC411559 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and ATG3-Knockout 293T cells (KO, Cat# LC812022) were separated by SDS-PAGE and immunoblotted with anti-ATG3 monoclonal antibody TA503370, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503370)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503370)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503370)

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  Anti-ATG3 mouse monoclonal antibody (TA503370) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3 (RC203453).

• HEK293T cells transfected with either RC203453 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATG3 antibody (TA503370), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4F6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.7 kDa
• Gene Name: Homo sapiens autophagy related 3 (ATG3), transcript variant 1, mRNA.
• Database Link:
  NP_071933
  Entrez Gene 67841 MouseEntrez Gene 171415 RatEntrez Gene 64422 Human
  UniProt ID Q9NT62
• Background: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]). [supplied by OMIM]
• Synonyms: APG3; APG3-LIKE; APG3L; PC3-96

Reference Data

• Protein Pathways: Regulation of autophagy