FMRP (FMR1) Mouse Monoclonal Antibody [Clone ID: OTI1H9]

CAT#: CF504342

Carrier-free (BSA/glycerol-free) FMR1 mouse monoclonal antibody, clone OTI1H9 (formerly 1H9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FMR1 (RC222699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FMR1. Positive lysates LY419580 (100ug) and LC419580 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of permeabilized Hela cells, using anti-FMR1 antibody (TA504342), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

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  Flow cytometric Analysis of permeabilized Jurkat cells, using anti-FMR1 antibody (TA504342), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Specifications

Product Data

• Clone Name: OTI1H9
• Applications: FC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 36-279 of human FMR1(NP_002015) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71 kDa
• Gene Name: Homo sapiens fragile X mental retardation 1 (FMR1), transcript variant ISO1, mRNA.
• Database Link:
  NP_002015
  Entrez Gene 14265 MouseEntrez Gene 24948 RatEntrez Gene 2332 Human
  UniProt ID Q06787
• Background: The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene. [provided by RefSeq]
• Synonyms: FMRP; FRAXA; POF; POF1; POFX

Reference Data

• Protein Families: Druggable Genome