Carboxypeptidase A (CPA1) Mouse Monoclonal Antibody [Clone ID: OTI4C3]

CAT#: CF504519

Carrier-free (BSA/glycerol-free) CPA1 mouse monoclonal antibody, clone OTI4C3 (formerly 4C3)

Product Images

Anti-CPA1 mouse monoclonal antibody (TA504519) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CPA1 (RC202720).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CPA1 (RC202720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CPA1. Positive lysates LY419696 (100ug) and LC419696 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC202720 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CPA1 antibody (TA504519), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-CPA1 antibody (TA504519), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-CPA1 antibody (TA504519), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4C3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CPA1(NP_001859) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.5 kDa
• Gene Name: Homo sapiens carboxypeptidase A1 (CPA1), mRNA.
• Database Link:
  NP_001859
  Entrez Gene 1357 Human
  UniProt ID P15085
• Background: Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a monomeric pancreatic exopeptidase involved in zymogen inhibition. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: CPA

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Secreted Protein