MIF4GD Mouse Monoclonal Antibody [Clone ID: OTI2H9]

CAT#: CF504558

Carrier-free (BSA/glycerol-free) MIF4GD mouse monoclonal antibody, clone OTI2H9 (formerly 2H9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIF4GD (RC207489, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIF4GD. Positive lysates LY402799 (100ug) and LC402799 (20ug) can be purchased separately from OriGene.

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  Anti-MIF4GD mouse monoclonal antibody (TA504558) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MIF4GD (RC207489).

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  HEK293T cells transfected with either RC207489 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MIF4GD antibody (TA504558), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2H9
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MIF4GD(NP_065730) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.2 kDa
• Gene Name: Homo sapiens MIF4G domain containing (MIF4GD), transcript variant 2, mRNA.
• Database Link:
  NP_065730
  Entrez Gene 69674 MouseEntrez Gene 360659 RatEntrez Gene 57409 Human
  UniProt ID A9UHW6
• Background: This gene encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3' end of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
• Synonyms: AD023; MIFD; SLIP1