ACSF2 Mouse Monoclonal Antibody [Clone ID: OTI6E4]

CAT#: CF504711

Carrier-free (BSA/glycerol-free) ACSF2 mouse monoclonal antibody, clone OTI6E4 (formerly 6E4)

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACSF2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504711)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ACSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504711)

• 

  Anti-ACSF2 mouse monoclonal antibody (TA504711) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACSF2 (RC204368).

• 

  HEK293T cells transfected with either RC204368 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACSF2 antibody (TA504711), and then analyzed by flow cytometry.

Show all 5 images

Specifications

Product Data

• Clone Name: OTI6E4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACSF2(NP_079425) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67.9 kDa
• Gene Name: Homo sapiens acyl-CoA synthetase family member 2 (ACSF2), transcript variant 2, mRNA.
• Database Link:
  NP_079425
  Entrez Gene 80221 Human
  UniProt ID Q96CM8
• Synonyms: ACSMW; AVYV493