DCC Mouse Monoclonal Antibody [Clone ID: OTI4A1]

CAT#: CF804515

Carrier-free (BSA/glycerol-free) DCC mouse monoclonal antibody, clone OTI4A1 (formerly 4A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCC (RC218506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCC. Positive lysates LY417439 (100ug) and LC417439 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4A1
• Applications: WB
• Recommended Dilution: WB 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DCC (NP_005206) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 158.3 kDa
• Gene Name: Homo sapiens DCC netrin 1 receptor (DCC), mRNA.
• Database Link:
  NP_005206
  Entrez Gene 13176 MouseEntrez Gene 25311 RatEntrez Gene 1630 Human
  UniProt ID P43146
• Background: This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma. [provided by RefSeq, Oct 2009]
• Synonyms: CRC18; CRCR1; IGDCC1; MRMV1; NTN1R1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance, Colorectal cancer, Pathways in cancer