RAB3A Mouse Monoclonal Antibody [Clone ID: OTI5B9]

CAT#: CF809533

Carrier-free (BSA/glycerol-free) RAB3A mouse monoclonal antibody,clone OTI5B9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB3A (RC203973, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAB3A (1:2000). Positive lysates LY419059 (100ug) and LC419059 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5B9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RAB3A(NP_002857) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens RAB3A, member RAS oncogene family (RAB3A), mRNA.
• Database Link:
  NP_002857
  Entrez Gene 5864 Human
  UniProt ID P20336
• Background: Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. [UniProtKB/Swiss-Prot Function]

Reference Data

• Protein Families: Druggable Genome