MLH1 Mouse Monoclonal Antibody [Clone ID: OTI3C1]

CAT#: CF810261

Carrier-free (BSA/glycerol-free) MLH1 mouse monoclonal antibody,clone OTI3C1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLH1 (RC201607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLH1 (1:2000). Positive lysates LY400096 (100ug) and LC400096 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-MLH1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810261) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810261) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810261) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810261) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810261) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810261) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810261) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MLH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810261) (1:1000)

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Specifications

Product Data

• Clone Name: OTI3C1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 299-527 of human MLH1(NP_000240) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.4 kDa
• Gene Name: Homo sapiens mutL homolog 1 (MLH1), transcript variant 1, mRNA.
• Database Link:
  NP_000240
  Entrez Gene 17350 MouseEntrez Gene 81685 RatEntrez Gene 4292 Human
  UniProt ID P40692
• Background: This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined. [provided by RefSeq, Nov 2009]
• Synonyms: COCA2; FCC2; hMLH1; HNPCC; HNPCC2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer