TEDC1 Mouse Monoclonal Antibody [Clone ID: OTI6A3]

CAT#: CF811468

Carrier-free (BSA/glycerol-free) C14orf80 mouse monoclonal antibody,clone OTI6A3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C14orf80 (RC203670, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C14orf80 (1:2000).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-C14orf80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811468) (1:250)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-C14orf80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811468) (1:250)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-C14orf80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811468) (1:250)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-C14orf80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811468) (1:250)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C14orf80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811468) (1:250)

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Specifications

Product Data

• Clone Name: OTI6A3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:250
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C14ORF80 (NP_775879) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens chromosome 14 open reading frame 80 (C14orf80), transcript variant 3
• Database Link:
  NP_775879
  Entrez Gene 283643 Human
  UniProt ID Q86SX3
• Synonyms: chromosome 14 open reading frame 80; MGC16771