UBE2NL Mouse Monoclonal Antibody [Clone ID: OTI8E6]

CAT#: CF812448

Carrier-free (BSA/glycerol-free) UBE2NL mouse monoclonal antibody,clone OTI8E6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2NL (RC215865, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2NL (1:500). Positive lysates LY422948 (100ug) and LC422948 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI8E6
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-100 of human UBE2NL (NP_001013007) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.2 kDa
• Gene Name: Homo sapiens ubiquitin conjugating enzyme E2 N like (gene/pseudogene) (UBE2NL), transcript variant coding, mRNA.
• Database Link:
  NP_001013007
  Entrez Gene 389898 Human
  UniProt ID Q5JXB2
• Background: This gene is intronless and encodes a member of the ubiquitin-conjugating enzyme family. The protein product is 91% identical to ubiquitin-conjugating enzyme E2N, a multi-exon gene product. This locus represents a polymorphic pseudogene, where some individuals contain an allele that can encode a full-length protein, while others have a non-functional allele containing a premature stop codon (reference SNP rs237520) that truncates the coding sequence. [provided by RefSeq, Jun 2014]
• Synonyms: Li174