MX1 Mouse Monoclonal Antibody [Clone ID: OTI2E7]

CAT#: CF812749

Carrier-free (BSA/glycerol-free) MX1 mouse monoclonal antibody,clone OTI2E7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MX1 (RC207418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MX1 (1:500). Positive lysates LY400886 (100ug) and LC400886 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2E7
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MX1 (NP_002453) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 75.3 kDa
• Gene Name: Homo sapiens MX dynamin like GTPase 1 (MX1), transcript variant 2, mRNA.
• Database Link:
  NP_002453
  Entrez Gene 4599 Human
  UniProt ID P20591
• Background: This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
• Synonyms: IFI-78K; IFI78; MX; MxA

Reference Data

• Protein Families: Druggable Genome