PDZK1 Mouse Monoclonal Antibody [Clone ID: OTI2B4]

CAT#: CF813142

Carrier-free (BSA/glycerol-free) PDZK1 mouse monoclonal antibody,clone OTI2B4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDZK1 (RC200444, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDZK1 (1:2000).

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  Western blot analysis of extracts (35ug) from 1 cell lines lysates by using anti-PDZK1 monoclonal antibody (1:250).

Specifications

Product Data

• Clone Name: OTI2B4
• Applications: WB
• Recommended Dilution: WB 1:250~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDZK1 (NP_002605) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.9 kDa
• Gene Name: Homo sapiens PDZ domain containing 1 (PDZK1), transcript variant 1, mRNA.
• Database Link:
  NP_002605
  Entrez Gene 5174 Human
  UniProt ID Q5T2W1
• Background: This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
• Synonyms: CAP70; CLAMP; NHERF-3; NHERF3; PDZD1