Basophils Mouse Monoclonal Antibody [Clone ID: 212H6.16]

CAT#: DDX0030B-100

Basophils mouse monoclonal antibody, clone 212H6.16

Product Images

IHC staining of human skin frozen section with clone 212H6 (DDX0030) (psoriasis)

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  Giemsa staining of Macs-isolated 212H6+ cells. Absence of degranulation

Specifications

Product Data

• Clone Name: 212H6.16
• Applications: FC, IF
• Recommended Dilution: IHC, WB, Flow Cytometry.
  Applications tested: MACS- separation and flow cytometry, IHC frozen section.
  Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: enriched-human tonsillar pDCs
• Specificity: Human basophils and mast cells.
  Species cross-reactivity: no-cross reactivity on mouse basophil.
• Formulation: Label: Biotin
  State: 100 μg in 200 μl/ 50 μg in 100 μl PBS 50% glycerol
• Concentration: 0.5 mg/ml
• Purification: QMA Hyper D Ion exchange chromatography
• Conjugation: Biotin
• Background: Basophils and mast cells are multifunctional hematopoietic effector cells involved in allergic and inflammatory reactions. Immediate allergy is caused by release of chemical mediators by these cell types. In human peripheral blood, 212H6.16 is an antibody specific to a yet non-identified cell-surface structure (~30KDa in reduced WB conditions and ~46KDa in non-reduced WB conditions) present on basophils. 212H6.16 does not induce basophil degranulation, and is therefore a valuable tool for the isolation and study of human basophils. Tonsillar epithelium and skin keratinocytes are also stained by 212H6 in situ: antigen expression in tonsil is restricted to cells lining the epithelial crypts.
  (Bendriss-Vermare N.et al, Leukocyte Typing VII, White Cell Differentiation Antigen MC13, part 9, myeloid cells, 425-30, 2001; Editor Mason)
• Synonyms: Basophil cells