CD40L (CD40LG) Mouse Monoclonal Antibody [Clone ID: LL48]

CAT#: DDX0140P-100

CD40L (CD40LG) mouse monoclonal antibody, clone LL48

Product Images

IF staining of hCD40L-transfected L cells with LL48 clone

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  Inhibition of IL12 production by CD40L-stimulated humanmonocyte-derivedDCs by LL48 clone (Stax AM et al, 2008, J ImmunolMethods)

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  FACS staining of hCD40L-transfected L cells with LL48 clone

Specifications

Product Data

• Clone Name: LL48
• Applications: FC, FN, IF
• Recommended Dilution: Cell culture inhibition in MELR H3, Thymidine uptake proliferative assay Immunofluorescence.
  Flow cytometry, neutralization of CD40L biological effects.
  *This monoclonal antibody may be used between 5-20 μg/ml.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Chimeric CD40L-Ig-transfected COS-7 cells.
• Specificity: Human CD40L.
• Formulation: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
  State: Purified
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD40 ligand
• Database Link:
  Entrez Gene 959 Human
  UniProt ID P29965
• Background: CD40 ligand also known as TRAP, gp39, TNFSF5, is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF superfamily. CD40 ligand is expressed predominantly on activated CD4+ T cells and is also found on other cell types, including NK cells, mast cells, basophils and eosinophils. Genetic alterations in the CD40L results in the absence of a functional molecule on activated T cells and are responsible for the occurrence of X-linked hyper-IgM syndrome. LL48 clone is a CD40L neutralizing antibody and plays an important role in costimulatory interactions between T and B lymphocytes, and antigen-presenting cells and lymphocytes regulating the immune system at multiple levels.
  (Tong AW et al, 2001, Clin. Canc. Res., 7:691-703; Hill A. et al, 1993; Nature, 361:494-503; Péguet- Navarro J. et al, 1995; J. Immunol., 155:4241-4247).
• Synonyms: CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39