DC SIGN (CD209) Mouse Monoclonal Antibody [Clone ID: 111H2.02]

CAT#: DDX0209B-100

DC SIGN (CD209) mouse monoclonal antibody, clone 111H2.02

Product Images

IHC staining of formalin fixed and paraffin-embedded human skin sections wtih 111H2.02

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  FACS staining of NIH3T3 transfected cells with 111H2.02

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  Endogenous expression revealed by FACS staining with 111H2.02

Specifications

Product Data

• Clone Name: 111H2.02
• Applications: FC, IHC
• Recommended Dilution: DDX0209P-50 / DDX0209P-100 purified: Surface flow cytometry, IHC Formol- paraffin, Bouin paraffin, Histowax.
  DDX0209A488-50 / DDX0209A488-100 Alexa-Fluor®488: Surface Flow cytometry.
  DDX0209A546-50 / DDX0209A546-100 Alexa-Fluor®647: IF.
  DDX0209A647-50 / DDX0209A647-100 Alexa-Fluor®647: Surface Flow cytometry.
  DDX0209B-50 DDX0209B-100 Biotin (on request): Surface Flow cytometry, IHC.
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: HeLa cells stably transfected-with human DC-SIGN.
• Specificity: Human DC-SIGN.
  Species cross-reactivity: positive staining on NIH3T3-transfected LSIGN.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Biotin
• Concentration: lot specific
• Conjugation: Biotin
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: CD209 molecule
• Database Link:
  Entrez Gene 30835 Human
  UniProt ID Q9NNX6
• Background: DC-SIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”)/CD209 and liver/lymph node-specific ICAM-3-grabbing nonintegrin (L-SIGN) (CD299/DC-SIGNR for DC-SIGN-related molecule; DC-SIGN2) are closely related genes that map to chromosome 19p13.3.Both genes encode a member of the C-type lectin family of type II transmembrane proteins. The two receptors are 77% identical at the amino acid level, have similar ligands. They are expressed in different tissues. Both receptors have been shown to interact with ICAM-3 DC-SIGN is a high affinity receptor for HIV gp120 (Soilleux EJ. 2003, Clinical Science 104, 437-; Dakappagari N., et al. 2006, The J Immunol, 176, 426; Geijtenbeeck T.B., et al. 2000, Cell, 100, 57 ; .Bashirova A. et al., 2001, J.Exp. Med., 193, 671). The clone 111H2.02 anti-DC-SIGN blocks HIVgp120-DC-SIGN interaction and reacts on formalin-fixed tissues (Canard B et al, Immunol Lett. 2011, 135(1-2):165-72).
• Synonyms: DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker