Actin (ACTA1) Mouse Monoclonal Antibody [Clone ID: 5C5]

CAT#: DM128-05

Actin (ACTA1) mouse monoclonal antibody, clone 5C5, Supernatant

Product Images

Formalin fixed paraffin embedded human skeletal muscle stained with sarcomeric Actin antibody (clone 5C5)

Specifications

Product Data

• Clone Name: 5C5
• Applications: IHC
• Recommended Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. Suitable for formalin/paraffin tissues.
  We suggest an incubation period of 30 minutes at room temperature.
  Recommended positive control: skeletal muscle.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Purified rabbit striated muscle actin
• Specificity: This antibody is specific to alpha-skeletal and alpha-cardiac muscle actins. Monoclonal anti-alpha-sarcomeric Actin has been used as a marker for rhabdomyosarcoma.
  Cellular localization: cytoplasmic.
• Formulation: State: Supernatant
  State: Diluted ascites containing sodium azide as preservative.
• Storage: Store the antibody at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 58 Human
• Background: The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
• Synonyms: ACTA; Alpha-actin-1; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3