TIE1 Mouse Monoclonal Antibody [Clone ID: 6F12]

CAT#: DM3517B

TIE1 mouse monoclonal antibody, clone 6F12, Biotin

Product Images

FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC). Upper panel: no primary antibody; Middle panel: solely conjugated secondary antibody; Lower panel: Biotin-conjugated anti-Human TIE-1.

Specifications

Product Data

• Clone Name: 6F12
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA: 1-15 μg/ml.
  Western blot: 1-2 μg/ml.
  FACS: 1-5 μg/ml.
  Cell sorting: 2-5 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human soluble extracellular TIE-1 protein.
• Specificity: This unconjugated monoclonal antibody will detect native TIE-1 in ELISA experiments and on the surface of different cell types.
• Formulation: PBS
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Stabilizer: BSA (50X)
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml
• Purification: Protein G Chromatography
• Conjugation: Biotin
• Database Link:
  Entrez Gene 7075 Human
• Background: The soluble receptor protein consists of the full extracellular domain (Met1-Glu749). The recombinant mature TIE-1/Fc is a disulfide-linked homodimeric protein. Human TIE-1/Fc monomer has a calculated molecular mass of approximately 105kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125kDa protein in SDS-PAGE under reducing conditions.TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region.
  These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.
• Synonyms: TIE, Tie-1