Macrophage inflammatory protein 5 (CCL15) Goat Polyclonal Antibody
CAT#: PP1088P1
Macrophage inflammatory protein 5 (CCL15) goat polyclonal antibody, Aff - Purified
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Specifications
Product Data | |
Applications | ELISA, FN, WB |
Recommended Dilution | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-5 (100.00 ng/ml), a concentration of 2.0-3.0 µg/ml of this antibody is required. ELISA: To detect hMIP-5 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hMIP-5. Western Blot: To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivities | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Highly pure (>98%) recombinant hMIP-5. |
Specificity | Macrophage Inflammatory Protein-5 (MIP-5). |
Formulation | PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity chromatography. |
Storage | Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Database Link | |
Background | Macrophage inflammatory protein 5 (CCL15) is a member of the human beta-chemokine family and is chemotactic for T cells and monocytes but not neutrophils, eosinophils, or B cells. It induces changes in intracellular calcium concentration in monocytes and is thought to act through the CCR1 receptor but also binds to CCR3. |
Synonyms | C-C motif chemokine 15, Small-inducible cytokine A15, Chemokine CC-2, NCC-3, NCC3, MIP-5, MIP-1 delta, Leukotactin-1, Mrp-2b, SCYA15 |
Note | Centrifuge vial prior to opening! |
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