Msr1 Rat Monoclonal Antibody [Clone ID: 2F8]

CAT#: SM029B

Msr1 rat monoclonal antibody, clone 2F8, Biotin

Product Images

Staining of mouse peritoneal macrophages with RAT ANTI MOUSE CD204: BIOTIN.

Specifications

Product Data

• Clone Name: 2F8
• Applications: FC
• Recommended Dilution: Flow cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
  The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: RAW264 cell line.
  Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line.
• Specificity: This antibody recognises the murine Scavenger Receptor Class A (SRA), type I and II, also known as CD204.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer
  Label: Biotin
  State: Liquid purified IgG fraction
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G
• Conjugation: Biotin
• Database Link:
  Entrez Gene 20288 Mouse
• Background: CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion.
  Recent research shows that clone 2F8 recognises an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain.
• Synonyms: MSR1, SCARA1