Integrin alpha 6 (ITGA6) Rat Monoclonal Antibody [Clone ID: NKI-GoH3]

CAT#: SM038F

Integrin alpha 6 (ITGA6) rat monoclonal antibody, clone NKI-GoH3, FITC

Product Images

Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN/MOUSE CD49f:FITC

Specifications

Product Data

• Clone Name: NKI-GoH3
• Applications: FC
• Recommended Dilution: Flow cytometry: Use 10 ul of neat antibody to label 10e6 cells in 100 ul.
• Reactivities: Canine, Human, Monkey, Mouse, Porcine, Sheep
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.
• Specificity: This antibody recognises CD49F (VLA-6 alpha chain). Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts. VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin.
• Formulation: PBS containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha 6
• Database Link:
  Entrez Gene 3655 Human
• Background: CD49f is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells.
• Synonyms: Integrin alpha-6, VLA-6, VLA6