CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: AT10]
Specifications
Product Data | |
Clone Name | AT10 |
Applications | FC |
Recommended Dilution | Flow Cytometry: Use 10 µl of neat antibody to label 106 cells or 100 µl whole blood. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | K562 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line. |
Specificity | This antibody recognizes the CD32 antigen. Clone AT10 blocks the binding of IgG to Fc gamma R11. SM1092LE is recommended for this purpose. |
Formulation | PBS, pH 7.4 Label: Biotin State: Liquid purified IgG fraction. Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Biotin |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | CD32 is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. |
Synonyms | Fc-gamma RII-a, Fc-gamma-RIIa, CDw32, FCGR2A, FCG2, FCGR2A1, IGFR2 |
Reference Data |
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