EGFR Rat Monoclonal Antibody [Clone ID: ICR10]

CAT#: SM1334P

EGFR rat monoclonal antibody, clone ICR10, Purified

Product Images

Staining of A431 cells with RAT ANTI HUMAN EGF RECEPTOR visualised with F (ab')2 RABBIT ANTI RAT IgG:FITC.

Specifications

Product Data

• Clone Name: ICR10
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Western blotting.
  Flow cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
  Immunohistochemistry on frozen sections.
  Recommended positive control: Breast carcinoma.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Extracellular domain of human Epidermal Growth Factor Receptor (EGF R) from head and neck carcinoma.
• Specificity: This antibody recognises the epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas. High level expression of EGFR is often associated with advanced disease and poor prognosis. This antibody binds to epitope B from EGFR (1, 2) and has an affinity of 6.7 x 10e-9 M.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Ion exchange chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1956 Human
• Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
• Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1