alpha Defensin 1 (DEFA1) Mouse Monoclonal Antibody [Clone ID: DEF-3]

CAT#: SM1382

alpha Defensin 1 (DEFA1) mouse monoclonal antibody, clone DEF-3, Purified


USD 675.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name DEF-3
Applications IHC
Recommended Dilution Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/1000).
Immunohistochemistry on Paraffin Sections: 0.5 µg/ml (1/400), Proteinase K pretreatment for antigen retrieval is recommended.
Positive Control: Human tonsil.
Has been discribed to work in FACS with permeabilized cells.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Native defensins.
The epitope has not been further characterized.
Specificity Several Defensins are recognized.
This antibody recognises a family of Cyclic Peptides in Neutrophils.
Human:
Defensin 1-3 (HNP-1 to HNP-3) in Human Neutrophils.
Synthetic Defensin-1 and -2 (Bachem, Bubendorf CH) stain also positively. Not tested with Defensin-4. A side reaction to elastin has been observed in humans.
Other: not tested. Defensins have been described in rabbits (NP-1 to NP-5, NP3a = Corticostatin), and in mice (cryptodin).
Formulation PBS, pH 7.2, containing 5 mg/ml BSA and 0.09% sodium azide
State: Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Restore with 0.5 ml distilled water.
Concentration 0.2 mg/ml (after reconstitution)
Purification Affinity chromatography
Storage Store the original vial at 2-8°C.
Freeze stock solution or aliquots thereof at -20°C.
Avoid repeated freezing and thawing.
Do not freeze working dilutions.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens defensin alpha 1B (DEFA1B), transcript variant 2
Background Defensins are a family of cyclic peptides containing 29-35 amino acids (MW < 3500) which tend to form aggregates. Four of these peptides are described in humans (HNP-1 to 4), six in rabbits (NP-1 to 5). Synonyms are MCP-1 for NP-1, MCP-2 for NP-2 and corticostatin for NP-3.
Biological Functions: Various functions have been described for defensins. They are antibacterial, antifungal, chemotactic for monocytes, inhibitory for ACTH-induced corticosteroid synthesis and cytotoxic for cells. Defensins are inhibited by glucosaminoglycans (self protection for cells) and high concentrations of Ca2+ ions.
Synonyms DEFA1, DEF-A1, DEFA-1, DEF1, DEFA2, MRS, DEF-1, DEFA-2, DEF-A2, HNP-1, HP-1, HP1, HNP1, Neutrophil defensin 1, DEFA3, DEFA-3, DEF-A3, DEF3, DEF-3, HNP-3, HP-3, HP3, HNP3, Neutrophil defensin 3, Defensin alpha 1, Defensin alpha 3
Reference Data
Protein Families Druggable Genome

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.