TRAIL (TNFSF10) (95-281) Mouse Monoclonal Antibody [Clone ID: 2E5]

CAT#: SM1847LE

TRAIL (TNFSF10) (95-281) mouse monoclonal antibody, clone 2E5, Low Endotoxin

Product Images

Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5). The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL. A - medium B - recombinant TRAIL C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 ug/ml) D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 ug/ml) E - recombinant TRAIL + Isotype mouse IgG1 controlE - recombinant TRAIL + Isotype mouse IgG1 control

Specifications

Product Data

• Clone Name: 2E5
• Applications: Assay, FC, FN
• Recommended Dilution: Functional application: High neutralizing activity for human TRAIL in biological assays.
  Flow cytometry: 1-10 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant soluble fragment (aa 95-281) of human TRAIL
• Specificity: This antibody reacts with TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.
• Formulation: Buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
  State: Low Endotoxin
  State: Liquid Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein A (> 95% pure by SDS-PAGE)
• Database Link:
  Entrez Gene 8743 Human
• Background: Human CD253 / TRAIL (TNF-Related Apoptosis Inducing Ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2).
• Synonyms: Apo-2 ligand, TNFSF10, APO2L, Apo-2L