MADCAM1 Mouse Monoclonal Antibody [Clone ID: 314G8]

CAT#: SM2237B

MADCAM1 mouse monoclonal antibody, clone 314G8, Biotin

Specifications

Product Data

• Clone Name: 314G8
• Applications: ELISA, FC
• Recommended Dilution: Flow Cytometry: (Neat-1/10): Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
  ELISA: 1-10 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant soluble MAdCAM-1-fc fusion protein.
  Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
• Specificity: This antibody recognises mucosal addressin cell adhesion molecule 1 (MAdCAM-1). Clone 314G8 does not recognise MAdCAM-1 in the gut lamina propria. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7. We recommend the use of SM2237LE for this purpose.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin.
  Label: Biotin
  State: Liquid purified IgG fraction.
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Biotin
• Database Link:
  Entrez Gene 8174 Human
• Background: MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.
• Synonyms: hMAdCAM-1, MAdCAM-1, Mucosal addressin cell adhesion molecule 1