TRIM5 alpha (TRIM5) Rabbit Polyclonal Antibody

CAT#: TA306153

Rabbit Polyclonal TRIM5 alpha Antibody

Product Images

Western blot analysis of TRIM5 alpha expression in human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 ug /ml.

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  Immunofluorescence of TRIM5 alpha in human breast tissue with TRIM5 alpha antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 2 ug/mL, IF: 10 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5 alpha.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tripartite motif containing 5
• Database Link:
  NP_149023
  Entrez Gene 85363 Human
  UniProt ID Q9C035
• Background: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region (1). TRIM5 has six alternately spliced isoforms, the longest of which is the ? variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1 (2,3), albeit at differing efficiencies. All TRIM5alpha variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5alpha acts as a natural barrier to cross-species retrovirus transmission (3).
• Synonyms: RNF88; TRIM5alpha

Reference Data

• Protein Families: Druggable Genome