GLO1 Rabbit Polyclonal Antibody

CAT#: TA308044

Rabbit Polyclonal antibody to Glyoxalase I (glyoxalase I)

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. TA308044 diluted at 1:1000.

• 

  Sample (30ug whole cell lysate). A: 293T. B: A431. C: H1299. D: HeLa S3. E: Hep G2. F: MOLT4. G: Raji. 12% SDS PAGE. TA308044 diluted at 1:1000

• 

  Immunohistochemical analysis of paraffin-embedded OVCA, using GLO1 (TA308044) antibody at 1:100 dilution.

• 

  Glyoxalase I antibody [N1C3] detects GLO1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GLO1 protein stained by Glyoxalase I antibody [N1C3] (TA308044) dilute

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 184 of Glyoxalase I (Uniprot ID#Q04760)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21 kDa
• Gene Name: glyoxalase I
• Database Link:
  NP_006699
  Entrez Gene 109801 MouseEntrez Gene 294320 RatEntrez Gene 2739 Human
  UniProt ID Q04760
• Background: The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq]
• Synonyms: GLOD1; GLYI; HEL-S-74
• Note: Seq homology of immunogen across species: Rat (91%), Xenopus laevis (82%), Bovine (90%), Xenopus tropicalis (82%)

Reference Data

• Protein Pathways: Pyruvate metabolism