EED Rabbit Polyclonal Antibody

CAT#: TA308744

Rabbit polyclonal antibody to EED (embryonic ectoderm development)

Product Images

EED antibody detects EED protein by Western blot analysis. A. 30 ug H1299 whole cell lysate/extract. B. 30 ug HCT116 whole cell lysate/extract. 10 % SDS-PAGE. EED antibody (TA308744) dilution: 1:1000

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  Immunohistochemical analysis of paraffin-embedded SAS Xenograft, using EED (TA308744) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed A431, using EED (TA308744) antibody at 1:500 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 229 of EED (Uniprot ID#O75530)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: embryonic ectoderm development
• Database Link:
  NP_694536
  Entrez Gene 13626 MouseEntrez Gene 293104 RatEntrez Gene 8726 Human
• Background: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
• Synonyms: HEED; WAIT1
• Note: Seq homology of immunogen across species: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%)

Reference Data

• Protein Families: Druggable Genome, Transcription Factors