EMA (MUC1) Rabbit Polyclonal Antibody

CAT#: TA312008

Rabbit polyclonal CD227/MUC1 (Tyr1229) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using CD227/MUC1 (Phospho-Tyr1229) antibody.The lane on the right is treated with the synthesized peptide.

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  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody.The picture on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of HepG2 cells, using CD227/MUC1 (Phospho-Tyr1229) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:1000
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human CD227/MUC1 around the phosphorylation site of tyrosine 1229 (S-P-YP-E-K)
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: mucin 1, cell surface associated
• Database Link:
  NP_002447
  Entrez Gene 4582 Human
  UniProt ID P15941
• Synonyms: ADMCKD; ADMCKD1; CA 15-3; CD227; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; SEC
• Note: CD227/MUC1 (Phospho-Tyr1229) antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane