Esrp2 Mouse Monoclonal Antibody [Clone ID: 23A7.C9]

CAT#: TA319605

Mouse monoclonal Esrp-1/2 antibody

Product Images

Anti-Esrp-1/2 antibody by WB shows detection in 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature.

Specifications

Product Data

• Clone Name: 23A7.C9
• Applications: WB
• Recommended Dilution: ELISA: 1:750,000, WB: 1:1000
• Reactivities: Mouse
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: epithelial splicing regulatory protein 2
• Database Link:
  NP_789808
  Entrez Gene 77411 Mouse
  UniProt ID Q8K0G8
• Synonyms: FLJ21918; FLJ22248; RBM35B
• Note: Epithelial splicing regulatory protein-1 (Esrp-1) and Esrp-2 are mRNA splicing factors that regulate the formation of epithelial cell-specific isoforms. They specifically regulate the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 and -2 act by directly binding specific sequences in mRNAs. They bind the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.