Esrp1 Mouse Monoclonal Antibody [Clone ID: 27H12]

CAT#: TA319607

Mouse monoclonal Esrp-1 antibody

Product Images

Anti-ESRP-1 by WB shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution and followed reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature.

Specifications

Product Data

• Clone Name: 27H12
• Applications: WB
• Recommended Dilution: ELISA: 1:750,000, WB: 1:1000
• Reactivities: Mouse
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: epithelial splicing regulatory protein 1
• Database Link:
  NP_918944
  Entrez Gene 207920 Mouse
  UniProt ID Q3US41
• Synonyms: FLJ20171; RBM35A; RMB35A
• Note: Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.