PKM2 (PKM) Rabbit Polyclonal Antibody

CAT#: TA322979

Anti-PKM2 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 30 μg Lane: Hela cells Primary antibody: TA322979 (PKM Antibody) at dilution 1/450 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds

• 

  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA322979 (PKM Antibody) at dilution 1/50 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA322979 (PKM Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Hela cells
  IHC: 50-200
  Positive control: Human cervical cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 11-221 amino acids of Human Pyruvate kinase isozymes M1/M2
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58 kDa
• Gene Name: pyruvate kinase, muscle
• Database Link:
  NP_002645
  Entrez Gene 18746 MouseEntrez Gene 25630 RatEntrez Gene 5315 Human
  UniProt ID P14618
• Background: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP; generating ATP and Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein; a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells; suggesting a role of this protein in bacterial pathogenesis.
• Synonyms: CTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, Purine metabolism, Pyruvate metabolism, Type II diabetes mellitus