CD95 (FAS) Rabbit Polyclonal Antibody

CAT#: TA323754

Anti-FAS Rabbit Polyclonal Antibody

Product Images

Gel: 10+12%SDS-PAGE Lysate: 40 μg Lane: Human fetal brain tissue Primary antibody: TA323754 (FAS Antibody) at dilution 1/550 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323754 (FAS Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323754 (FAS Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Human fetal brain tissue
  IHC: 25-100
  Positive control: Human colon cancer
  Predicted cell location: Secreted, Cell membrane
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 156-335 amino acids of human Fas (TNF receptor superfamily, member 6)
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38 kDa
• Gene Name: Fas cell surface death receptor
• Database Link:
  NP_000034
  Entrez Gene 14102 MouseEntrez Gene 246097 RatEntrez Gene 355 Human
  UniProt ID P25445
• Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death; and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD); caspase 8; and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade; and leads to apoptosis. This receptor has been also shown to activate NF-kappaB; MAPK3/ERK1; and MAPK8/JNK; and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described; some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
• Synonyms: ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; TNFRSF6

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Allograft rejection, Alzheimer's disease, Apoptosis, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Pathways in cancer, Type I diabetes mellitus