RBPJK (RBPJ) Rabbit Polyclonal Antibody

CAT#: TA324617

Rabbit polyclonal RBPJ Antibody (N-term)

Product Images

RBPJ Antibody (N-term) (Cat. #TA324617) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the RBPJ antibody detected the RBPJ protein (arrow).

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  RBPJ Antibody (N-term) (TA324617)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBPJ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of A2058 cell stained with RBPJ Antibody (N-term) (Cat#TA324617). A2058 cells were incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). RBPJ immunoreactivity is localized to nucleus significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, IF: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This RBPJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human RBPJ.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55637 Da
• Gene Name: recombination signal binding protein for immunoglobulin kappa J region
• Database Link:
  NP_005340
  Entrez Gene 3516 Human
  UniProt ID Q06330
• Synonyms: AOS3; CBF1; csl; IGKJRB; IGKJRB1; KBF2; RBP-J; RBPJK; RBPSUH; SUH

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Notch signaling pathway