HIF1AN Mouse Monoclonal Antibody [Clone ID: FIH 162c]

CAT#: TA326397

Mouse monoclonal FIH Antibody

Product Images

Western blot analysis of FIH in a human cell line mix, using a 1:1000 dilution of the antibody

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  FIH visualized using the antibody

Specifications

Product Data

• Clone Name: FIH 162c
• Applications: IHC, WB
• Recommended Dilution: WB: 10ug/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human FIH expressed in E.coli BL21 (DE3) cells
• Formulation: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Concentration: lot specific
• Purification: Protein G Purified
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: hypoxia inducible factor 1 alpha subunit inhibitor
• Database Link:
  NP_060372
  Entrez Gene 55662 Human
• Background: FIH, Factor inhibiting HIF1 (hypoxia-inducible factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional coactivators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia . Recent studies show that low nuclear expression of FIH is a strong independent prognostic factor for a poor overall survival in clear cell renal cell cancinoma .
• Synonyms: FIH1
• Note: Detects ~45kDa (human) proteins, corresponding to the molecular mass of FIH on SDS PAGE immunoblots

Reference Data

• Protein Families: Druggable Genome