Rad9 (RAD9A) Mouse Monoclonal Antibody [Clone ID: 93A535]

CAT#: TA336578

Mouse Monoclonal Rad9 Antibody (93A535)

Product Images

Immunohistochemistry-Paraffin: Rad9 Antibody (93A535) TA336578 - Formalin-fixed, paraffin-embedded human prostate adenocarcinoma stained with RAD9 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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  Western Blot: Rad9 Antibody (93A535) TA336578 - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test.

Specifications

Product Data

• Clone Name: 93A535
• Applications: IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunohistochemistry-Paraffin: 1:10-1:500, Western Blot: 1-2 ug/ml, Immunohistochemistry: 1:10-1:500, Immunoprecipitation: 1:10-1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: The antibody was raised against fusion protein of human full length Rad9.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RAD9 checkpoint clamp component A
• Database Link:
  NP_004575
  Entrez Gene 5883 Human
  UniProt ID Q99638
• Background: Rad9 is a recently isolated human protein that is involved in the control of cell cycle checkpoint. Human Rad9 (hRad9) is homologue of the S. pombe Rad9. Six Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are involved in the early steps of DNA damage check point response in S. pombe. Recently, it has been shown that hRad9 interacts with Bcl-2 family proteins Bcl-2 and Bcl-XL, but not with BAX or BAD. Over-expression of hRad9 in mammalian cells leads to apoptosis, where as antisense Rad9 suppresses cell death induced by DNA damaging agents such as methanosulphonate. Human Rad9 undergoes complex post-translational modifications in undamaged cells and is hyperphosphorylated in response to DNA damage and makes ternary complex with human Rad1 and human HUS1 proteins. This may account for detection of multiple protein bands in some cases by western blot or immunoprecipitation analysis using anti-hRad9 antibody. HRad9 has an intrinsic 3-5 exonuclease activity suggesting that by interaction with DNA it may play a role in the damage-sensing step of the DNA damage checkpoint pathway.
• Synonyms: RAD9
• Note: Immunocytochemistry/Immunofluorescence: see Kotmatsu et al. Immunohistochemistry-Paraffin: see Lee et al (2007) for details.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency