PDZK1 Rabbit Polyclonal Antibody

CAT#: TA336647

Rabbit Polyclonal PDZK1 Antibody

Product Images

Immunohistochemistry: PDZK1 Antibody TA336647 - IHC analysis of PDZK1 in mouse kidney using DAB with hematoxylin counterstain.

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  Immunocytochemistry/Immunofluorescence: PDZK1 Antibody TA336647 - PDZK1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

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  Simple Western: PDZK1 Antibody TA336647 - Simple Western lane view shows a specific band for PDZK1 in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

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  Western Blot: PDZK1 Antibody TA336647 - WB analysis of PDZK1 in fetal human liver lysate.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry: 1:400, Immunocytochemistry/ Immunofluorescence: 1:200, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:400, Simple Western: 1:250
• Reactivities: Human, Mouse, Bovine (Does not react with: Rat)
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to the C-terminus of human PDZK1. [UniProt# Q13113]
• Formulation: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57 kDa
• Gene Name: PDZ domain containing 1
• Database Link:
  NP_002605
  Entrez Gene 59020 MouseEntrez Gene 65144 RatEntrez Gene 5174 Human
  UniProt ID Q5T2W1
• Background: Scaffold or adaptor proteins recruits and/or anchor their binding partners to specific subcellular locations, serving as a platform to regulate interactions between proteins involved in diverse signal transduction pathways, and PDZK1 (PDZ domain-containing protein 1) is a scaffold protein belonging to NHERFs family. PDZK1 expression is largely limited to epithelial cells and is mainly expressed in renal proximal epithelial cells, hepatocytes, and at a lower level in other epithelial as well as certain endothelial cells. PDZK1 has four PDZ domains which facilitates its interaction with its binding partners, including ion transporters (e.g. CFTR, OCTN1/OCTN2 etc) and several GPCRs (e.g. HTR2B, SSTR family members). Through its interaction with HDL and SR-BI, PDZK1 can play role in reverse cholesterol transport and for HDL-mediated vascular re-endothelialisation. When complex with SLC9A3R1, PDZK1 cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking as well as the activity of associated membrane proteins. PDZK1 plays a role in cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. PDZK1 also implicates in connecting SCARB1 with cellular machineries for intracellular cholesterol transport and/or metabolism and regulation of proximal tubular Na+-dependent inorganic phosphate cotransport.
• Synonyms: CAP70; CLAMP; NHERF-3; NHERF3; PDZD1
• Note: This PDZK1 antibody is useful for Immunohistochemistry paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a single band is observed at ~70 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.