NFH (NEFH) Mouse Monoclonal Antibody [Clone ID: 9B12]

CAT#: TA336710

Mouse Monoclonal 200kDa Neurofilament Heavy Antibody (9B12)

Product Images

Western Blot: 200kDa Neurofilament Heavy Antibody (9B12) TA336710 - Strip blots of crude rat spinal cord extract stained with three different antibodies to phosphorylated NF-H, NB300-136 (lane 1), NBP1-05209 (Lane 2) and TA336710 (lane 3). All three ant

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  Immunocytochemistry/Immunofluorescence: 200kDa Neurofilament Heavy Antibody (9B12) TA336710 - Mixed neuron/glial cultures stained with TA336710 (red) and rabbit GFAP antibody NB300-141 (green). Axonal profiles are stained in red, while astrocytic cells

Specifications

Product Data

• Clone Name: 9B12
• Applications: IF, WB
• Recommended Dilution: WB: 1:10000, IF: 1:1000
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Native Neurofilament Heavy protein purified from bovine spinal cord
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 200 kDa
• Gene Name: neurofilament, heavy polypeptide
• Database Link:
  NP_066554
  Entrez Gene 24587 Rat
  UniProt ID P12036
• Background: Neuronal intermediate filaments (NIF) are found specifically on neurons where they play key role in maintaining neuronal morphology as well as in regenerating myelinated axons. NIF contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 200kD neurofilament heavy (NEFH) is one of the major components of neuronal cytoskeleton NIF and NEFH gene was originally proposed as a DNA marker for presymptomatic diagnosis in neurofibromatosis type 2 (NF2) . NEHF facilitate interactions between NIF and brain mitochondria with binding efficiency dependent upon mitochondrial membranes potential. NEFH and beta-catenin are constituents in postsynaptic density (PSD) in CNS and NEFH is essential for maintaining normal cell integrity. Diminished NEFH levels are seen in certain cancers including human autonomic nerve tumors or central neurocytomas, prostate cancer, clear-cell epithelioid tumor and small cell lung carcinoma. Fibroblasts overexpressing NEFH are distorted, multinucleated, and possess inclusions, typical changes seen in neuropathies, and defects in NEFH are linked to susceptibility to ALS.
• Synonyms: NFH
• Note: This 200kDa Neurofilament Heavy antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)