BRD2 Rabbit Polyclonal Antibody

CAT#: TA347084

Rabbit Polyclonal BRD2 Antibody

Product Images

WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Brd2, diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (predicted size: 88 kDa) is indicated on the right.

• 

  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Brd2. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,000.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (100) ; Western blotting (1:1,000)
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-BRD2 antibody: mouse Brd2 (bromodomain containing 2), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein (1).
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: bromodomain containing 2
• Database Link:
  NP_001106653
  Entrez Gene 14312 Mouse
  UniProt ID P25440
• Background: Brd2 (UniProtKB/Swiss-Prot entry P25440), a mitogen-activated kinase localized to the nucleus, is a putative transcriptional regulator which interacts with E2F1 and with histone H4 acetylated at 'Lys-13'. It is expressed during development and may be involved in growth control. Brd2 also may play a role in spermatogenesis or folliculogenesis and may be involved in some types of leukemia and in juvenile myoclonic epilepsy.
• Synonyms: D6S113E; FSH; FSRG1; NAT; RING3; RNF3

Reference Data

• Protein Families: Protein Kinase