Glucokinase (GCK) Rabbit Polyclonal Antibody

CAT#: TA350011

Rabbit Polyclonal Anti-GCK Antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: HT-29 cells
  IHC: 50-200
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human GCK
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52 kDa
• Gene Name: glucokinase
• Database Link:
  NP_000153
  Entrez Gene 24385 RatEntrez Gene 103988 MouseEntrez Gene 2645 Human
  UniProt ID P35557
• Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
• Synonyms: FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus