alpha Tubulin (TUBA1A) Mouse Monoclonal Antibody

CAT#: TA354368

Mouse anti Tubulin-a Monoclonal Antibody

Product Images

IHC: Human lung stained with Mouse anti-Tubulin-alpha antibody, at 1:100 for 30 min, RT. (Staining of formalin-fixed tissues requires boiling tissue section in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A recombinant protein of human Tubulin.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: tubulin alpha 1a
• Database Link:
  NP_001257329
  Entrez Gene 22142 MouseEntrez Gene 64158 RatEntrez Gene 7846 Human
  UniProt ID Q71U36
• Background: Tubulin is the major building block of microtubules which is essential in cell structures, transportation and mitosis. This superfamily is composed of six distinct families. Alpha, beta, and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. Each subunit is a 50 kDa acidic protein. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.
• Synonyms: B-ALPHA-1; LIS3; TUBA3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection