Aggrecan (ACAN) Rabbit Polyclonal Antibody

CAT#: TA354522

Rabbit anti Aggrecan/AGC1 Polyclonal Antibody

Product Images

WB: The tissue lysate derived from mouse embryonic tisse was resolved onto 7.5% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rb anti-Aggrecan at 1:500. multiple immunoreactive bands ~above 100 kDa was observed.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the 380-400 aa of human ACAN protein. This sequence is identical to bovine, mouse and rat.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: >200 kDa
• Gene Name: aggrecan
• Database Link:
  NP_001126
  Entrez Gene 11595 MouseEntrez Gene 58968 RatEntrez Gene 176 Human
  UniProt ID P16112
• Background: Aggrecan or aggregating chondroitin sulfate proteoglycan, is an integral part of the extracellular matrix in cartilagenous tissue. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight. The cleavage of Aggrecan has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration.
• Synonyms: AGC1; AGCAN; CSPG1; CSPGCP; MSK16; SEDK