UBE2D1 Rabbit Polyclonal Antibody

CAT#: TA356291

UBE2D1 Antibody - middle region

Product Images

WB Suggested Anti-UBE2D1 Antibody Titration: 1.25ug/ml Positive Control: Jurkat cell lysate

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  Lanes: 1: 40ng HIS-UBE2D1 protein 2: 40ng HIS-UBE2D2 protein 3: 40ng HIS-UBE2D3 protein 4: 40ng HIS-UBE2D4 protein 5: 40ng HIS-UBE2E1 protein 6: 40ng HIS-UBE2E2 protein 7: 40ng HIS-UBE2E3 protein 8: 40ng HIS-UBE2K protein 9: 40ng HIS-UBE2L3 protein 10: 40ng HIS-UBE2N protein 11: 40ng HIS-UBE2V1 protein 12: 40ng HIS-UBE2V2 protein. Primary Antibody Dilution: 1:500 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:50,000 Gene Name: UBE2D1 Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.

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  Host: Rat Target Name: UBE2D1 Sample Tissue: Rat Skeletal Muscle Antibody Dilution: 1ug/ml

Specifications

Product Data

• Applications: WB
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2D1
• Specificity: Expected reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
  Homology: Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 79%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 93%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Predicted Protein Size: 16kDa
• Gene Name: ubiquitin conjugating enzyme E2 D1
• Database Link:
  NP_001191809
  Entrez Gene 361831 RatEntrez Gene 7321 Human
  UniProt ID P51668
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
• Synonyms: E2(17)KB1; SFT; UBC4/5; UBCH5; UBCH5A

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis