IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: 143-13]

CAT#: TA363833

CD25 (IL-2 receptor alpha chain), mouse anti human, clone 143-13

Product Images

TA363833, Clone 143-13, human tonsil, frozen section

Specifications

Product Data

• Clone Name: 143-13
• Applications: IHC
• Recommended Dilution: Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 3µg/ml (1:130) Paraffin sections: not tested Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Stimulated human leukocytes.
• Specificity: Human: CD25 Other: not tested.
  
  Epitope: The antigen is CD25, a 55kD glycoprotein. The antibody recognizes epitope region C of the IL-2 receptor complex.
  
  Distribution: Isolated cells: The antibody stains less than 5% of resting human peripheral blood mononuclear cells in flow cytometry. Tissue sections: Scattered large and small lymphocytes in germinal centers, in follicle mantle, and T-zone.
• Formulation: Affinity purified, lyophilized Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA), and 0.09% sodium azide as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
• Gene Name: interleukin 2 receptor subunit alpha
• Database Link:
  Entrez Gene 3559 Human
  UniProt ID P01589
• Background: Monoclonal antibody 143-13 recognizes the CD25 antigen.CD25, the IL-2 receptor alpha chain, associates with CD122 and the common gamma chain CD132 to form the IL-2 receptor complex. It is expressed on activated T and B cells and on monocytes.
• Synonyms: CD25; IDDM10; IL-2-RA; IL2-RA; IL2R; p55; TCGFR