LAYN Rabbit Polyclonal Antibody

CAT#: TA366822

LAYN rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: Jurkat and K562 cell lysates Primary antibody: TA366822 (LAYN Antibody) at dilution 1/700 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 2 minutes

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA366822 (LAYN Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA366822 (LAYN Antibody) at dilution 1/50, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Jurkat and K562 cell lysates
  IHC: 50-100
  Positive control: Human esophagus cancer
  Predicted cell location: Cell membrane
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human LAYN
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 43 kDa
• Database Link:
  UniProt ID Q6UX15
• Background: Layilin is a recently cloned ∼55-kDa membrane-binding partner for talin (Borowsky and Hynes, 1998 ) that is widely expressed in different cell types and tissue extracts. It is found in peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells induced to migrate in in vitro wounding experiments. Layilin colocalizes with talin in ruffles and binds to talin's ∼50-kDa head domain (amino acids 280–435). Other parts of talin (the ∼220-kDa tail fragment) can bind β-integrin cytoplasmic tails, vinculin, and F-actin and hence form an integrin-cytoskeleton linkage at sites of cell-substratum contact. Layilin's extracellular domain is homologous with the carbohydrate-recognition domains (CRD) of C-type lectins. Receptor for hyaluronate.