CHD3 Rabbit Polyclonal Antibody

CAT#: TA367351

CHD3 rabbit polyclonal antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane: RAW264.7 cell lysate Primary antibody: TA367351 (CHD3 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 minutes

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  Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TA367351 (CHD3 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TA367351 (CHD3 Antibody) at dilution 1/50, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: RAW264.7 cell lysate
  IHC: 50-100
  Positive control: Human colorectal cancer
  Predicted cell location: Nucleus and Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human CHD3
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 226 kDa
• Database Link:
  UniProt ID Q12873
• Background: This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.