DOHH Rabbit Monoclonal Antibody [Clone ID: R04-4I8]

CAT#: TA384118

DOHH Rabbit monoclonal Antibody

Product Images

Western blot detection of DOHH in K562,Hela cell lysates using DOHH Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:33kDa.

Specifications

Product Data

• Clone Name: R04-4I8
• Applications: IP, WB
• Recommended Dilution: WB: 1/1000
  IP: 1/20
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human DOHH
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 33 kDa; Observed MW: 33 kDa
• Gene Name: deoxyhypusine hydroxylase/monooxygenase
• Database Link:
  Entrez Gene 83475 Human
  UniProt ID Q9BU89
• Background: Swiss-Prot Acc.Q9BU89.Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine (PubMed:16533814, PubMed:16371467, PubMed:19706422). Hypusination is unique to mature eIF-5A factor and is essential for its function .
• Synonyms: hDOHH; HLRC1; MGC4293