Histone H1.3 (HIST1H1D) Rabbit Monoclonal Antibody [Clone ID: R09-8H7]

CAT#: TA384359

Histone H1.3 Rabbit monoclonal Antibody

Product Images

Western blot detection of Histone H1.3 in C6,3T3,Hela cell lysates using Histone H1.3 Rabbit mAb(1:1000 diluted).Predicted band size:22kDa.Observed band size:30kDa.

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  Immunocytochemistry of Histone H1.3(green) in Hela cells using Histone H1.3 Rabbit mAb at dilution 1/50, and DAPI(blue)

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  Immunohistochemistry of Histone H1.3 in paraffin-embedded Human lung cancer tissue using Histone H1.3 Rabbit mAb at dilution 1/5

Specifications

Product Data

• Clone Name: R09-8H7
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/200
  ICC/IF: 1/100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Histone H1.3
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 22 kDa; Observed MW: 30 kDa
• Gene Name: histone cluster 1, H1d
• Database Link:
  Entrez Gene 3007 Human
  UniProt ID P16402
• Background: Swiss-Prot Acc.P16402.Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation .
• Synonyms: H1.3; H1F3; MGC138176