G CSF (CSF3) Mouse Monoclonal Antibody

CAT#: TA388903

G-CSF Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human G-CSF when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Human G-CSF as the detection antibody, at a concentration of approximately 0.5- 1.0 μg/ml.
  Western Blot
  To detect Human G-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human G-CSF is 1.5-2.0 ng/lane under non-reducing conditions, and 7.5-10.0 ng/lane under reducing conditions.
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Produced in BALB/c mice immunized with highly pure Recombinant Human G-CSF. Anti-Human G-CSF-specific antibody was purified from cell culture by Protein A affinity chromatography.
• Concentration: lot specific
• Purification: G-CSF-specific antibody was purified from cell culture by Protein A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: colony stimulating factor 3
• Database Link:
  Entrez Gene 1440 Human
  UniProt ID P09919
• Background: G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant Human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.
• Synonyms: filgrastim; G-CSF; GCSF; Lenograstim; MGC45931; pluripoietin